論文発表
小分子反応性ペプチドタグの指向性進化法による創製および生細胞内タンパク質イメージングと制御への応用
安東丈洋，横山匠，高守幸男，冨士大輔，川上隆史
色材協会誌（色材協会）, １２月号, pp331-338, 2025
SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling
Munshi AR, Wang T, Takamori Y, Ando T, Yokoyama T, Fuji D, Xu Z, Vedi S, Yamamoto M, Tsukamoto K, Kawakami T*.
Biosci Biotechnol Biochem. 88, 147-153, 2024
Growth optimization of single-phase novel colloidal perovskite Cs3Bi2I9 nanocrystals and Cs3Bi2I9@SiO2 core-shell nanocomposites for bio-medical application
Vedi S, Thangaraju D, Selvam G, Kawakami T, Rajeswaran N, Rajendran S, Muthukaruppan A, Alfaify S, Shkir M.
Biomater Sci. 10, 5956-5967, 2022
ペプチド医薬品開発のための新規ペプチド環状化法の開発と非天然型環状ペプチドSELEXへの応用
高守幸男，冨士大輔，安東丈洋，横山匠，佐藤将，ベディサンタナ，山本美月，川上隆史
バイオインダストリー（シーエムシー出版）, ７月号, pp9-17, 2022
Artificial aptamer that inhibits interleukin-23/interleukin-23 receptor interaction discovered via SELEX
Takamori Y, Ando T, Sato M, Vedi S, Fuji D, Yokoyama T, Tsukamoto K, Yamamoto M, Kawakami T*.
Biochem Biophys Res Commun. 614, 17-21, 2022
In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions
Yokoyama T, Ando T, Takamori Y, Fuji D, Sato M, Vedi S, Yamamoto M, Kawakami T*.
Chem Commun. 58, 5237-5240, 2022
Discovery of IL-5-binding unnatural cyclic peptides from multiple libraries by directed evolution
Fuji D, Ando T, Sato M, Vedi S, Takamori Y, Yokoyama T, Yamamoto M, Kawakami T*.
Biochem Biophys Res Commun. 610, 188-195, 2022
A human epidermal growth factor receptor 3/heregulin interaction inhibitor aptamer discovered using SELEX.
Yokoyama T, Ando T, Iwamoto R, Fuji D, Yamamoto M, Kawakami T*.
Biochem Biophys Res Commun. 553, 148-153, 2021
In vitro selection of an RNA aptamer yields an interleukin-6/interleukin-6 receptor interaction inhibitor.
Ando T, Yamamoto M, Takamori Y, Tsukamoto K, Fuji D, Kawakami T*.
Biosci Biotechnol Biochem. 85, 1170-1174, 2021
In vitro display evolution of IL-6R-binding unnatural peptides ribosomally initiated and cyclized with m-(chloromethyl)benzoic acid.
Takamori Y, Ando T, Fuji D, Yokoyama T, Yamamoto M, Kawakami T*.
Biochem Biophys Res Commun. 534, 27-33, 2021
Directed evolution of dibenzocyclooctyne-reactive peptide tags for protein labeling.
Ando T, Takamori Y, Yokoyama T, Yamamoto M, Kawakami T*.
Biochem Biophys Res Commun. 535, 47-53, 2021
In vitro display evolution of the PURE system-expressed TNFα-binding unnatural cyclic peptide containing an N-methyl-D-amino acid.
Tsukamoto K, Ando T, Fuji D, Yokoyama T, Takamori Y, Horiuchi D, Iwamoto R, Yamamoto M, Kawakami T*.
Biochem Biophys Res Commun. 534, 519-525, 2021
In vitro selection generates RNA aptamer that antagonizes PCSK9–LDLR interaction and recovers cellular LDL uptake.
Ando T, Yamamoto M, Yokoyama T, Horiuchi D, Kawakami T*.
J Biosci Bioeng. 131, 326-332, 2021
２種類の人工塩基を有するDNAアプタマー
山本美月, 川上隆史
実験医学（羊土社）, 1月号, pp62-63, 2018
細胞膜透過性ペプチド創製を指向したPUREシステムとmRNAディスプレイ法による大環状Nアルキルペプチドの超高速スクリーニング
山本美月, 清水優, 川上隆史
ペプチド医薬品開発のためのスクリーニング・安定化・製剤化技術（技術情報協会）, pp332-342, 2017
Directed Evolution of a Cyclized Peptoid-Peptide Chimera against a Cell-Free Expressed Protein and Proteomic Profiling of the Interacting Proteins to Create a Protein-Protein Interaction Inhibitor.
Kawakami T*, Ogawa K, Hatta T, Goshima N, Natsume T.
ACS Chem Biol. 11, 1569-77, 2016
DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells.
Kawakami T*, Ogawa K, Goshima N, Natsume T.
Chem Biol. 22, 1671-9, 2015
Incorporation of electrically charged N-alkyl amino acids into ribosomally synthesized peptides via post-translational conversion
Kawakami T*, Sasaki T, Reid PC, Murakami H
Chemical Science, 5, 887-893, 2014
Patch cloning method for multiple site-directed and saturation mutagenesis.
Taniguchi N, Nakayama S, Kawakami T, Murakami H.
BMC Biotechnol. 13, 91, 2013
Extensive reprogramming of the genetic code for genetically encoded synthesis of highly N-alkylated polycyclic peptidomimetics.
Kawakami T*, Ishizawa T, Murakami H.
J Am Chem Soc. 135, 12297-304, 2013
In vitro selection of multiple libraries created by genetic code reprogramming to discover macrocyclic peptides that antagonize VEGFR2 activity in living cells.
Kawakami T, Ishizawa T, Fujino T, Reid PC, Suga H, Murakami H.
ACS Chem Biol. 8, 1205-14, 2013
TRAP display: a high-speed selection method for the generation of functional polypeptides.
Ishizawa T, Kawakami T, Reid PC, Murakami H.
J Am Chem Soc. 135, 5433-40, 2013
Genetically encoded libraries of nonstandard peptides.
Kawakami T, Murakami H
J Nucleic Acids. 2012, 713510, 2012
Natural product-like macrocyclic N-methyl-peptide inhibitors against a ubiquitin ligase uncovered from a ribosome-expressed de novo library.
Yamagishi Y, Shoji I, Miyagawa S, Kawakami T, Katoh T, Goto Y, Suga H.
Chem Biol. 18, 1562-70, 2011
Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.
Jablonski AE1, Kawakami T, Ting AY, Payne CK.
J Phys Chem Lett. 1, 1312-1315, 2010
Diverse backbone-cyclized peptides via codon reprogramming.
Kawakami T, Ohta A, Ohuchi M, Ashigai H, Murakami H, Suga H.
Nat Chem Biol. 5, 888-90, 2009
Ribosomal synthesis of polypeptoids and peptoid-peptide hybrids.
Kawakami T, Murakami H, Suga H.
J Am Chem Soc. 130, 16861-3, 2008
Messenger RNA-programmed incorporation of multiple N-methyl-amino acids into linear and cyclic peptides.
Kawakami T, Murakami H, Suga H.
Chem Biol. 15, 32-42, 2008
A caged phosphopeptide-based approach for photochemical activation of kinases in living cells.
Kawakami T, Cheng H, Hashiro S, Nomura Y, Tsukiji S, Furuta T, Nagamune T
ChemBioChem. 9, 1583-6, 2008
Bone-like tissue formation by three-dimensional culture of MG63 osteosarcoma cells in gelatin hydrogels using calcium-enriched medium.
Takagishi Y, Kawakami T, Hara Y, Shinkai M, Takezawa T, Nagamune T.
Tissue Engineering. 12, 927-37, 2006
Effect of Copper Adsorption on the Mechanical Properties of Chitosan Beads
Mitani T, Kawakami T, Morishita M, Adachi Y, Ishii H
Journal of Applied Polymer Science. 88, 2988-2991, 2003